Undergraduate Thesis Or Project

Effect of Temperature on Osmotic Tolerance Limits for Adherent Endothelial Cells

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  • Cryopreservation depends on the equilibration of cells with high concentration of cryoprotective agents (CPAs). However, the addition of CPAs can induce damages to the cells. One of the damages is due to too much shrinkage or swell of the cell volume which lead to the cell death during the addition or removal of CPAs procedure. Therefore, it is important to optimize the tolerance limits of cell volume. The goal of this research is to investigate the effect of temperature on the tolerance limits of cell volume. The experiments are carried out with Bovine Pulmonary Artery Endothelial Cells (BPAEC), which are exposed to different NaCl treatment for fifteen minutes. The metabolic rates are later measured by the presto blue viability reagent. There are finding that at low temperature data there is a relatively high cell survival when exposing to extreme hypotonic or hypertonic solutions; and relatively low cell survival for the high temperature data set.
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  • Acknowledgement: Howard Hughes Medical Institute



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