Undergraduate Thesis Or Project
 

Effect of Temperature on Osmotic Tolerance Limits for Adherent Endothelial Cells

Público Deposited

Contenido Descargable

Descargar archivo
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/c821gm258

Descriptions

Attribute NameValues
Creator
Abstract
  • Cryopreservation depends on the equilibration of cells with high concentration of cryoprotective agents (CPAs). However, the addition of CPAs can induce damages to the cells. One of the damages is due to too much shrinkage or swell of the cell volume which lead to the cell death during the addition or removal of CPAs procedure. Therefore, it is important to optimize the tolerance limits of cell volume. The goal of this research is to investigate the effect of temperature on the tolerance limits of cell volume. The experiments are carried out with Bovine Pulmonary Artery Endothelial Cells (BPAEC), which are exposed to different NaCl treatment for fifteen minutes. The metabolic rates are later measured by the presto blue viability reagent. There are finding that at low temperature data there is a relatively high cell survival when exposing to extreme hypotonic or hypertonic solutions; and relatively low cell survival for the high temperature data set.
Resource Type
Fecha Disponible
Fecha de Emisión
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Non-Academic Affiliation
Declaración de derechos
Funding Statement (additional comments about funding)
  • Acknowledgement: Howard Hughes Medical Institute
Publisher
Language
Replaces

Relaciones

Parents:

This work has no parents.

En Collection:

Elementos

Miniatura Título Fecha de subida Visibilidad Acciones
file details Effect of Solute and Temperature on Osmotic Tolerance _Final.ppt 07/21/2017 Público
file details Effect of Solute and Temperature on Osmotic Tolerance _Final.pdf 07/21/2017 Público