Undergraduate Thesis Or Project

 

Look Ma, no beads: Analyzing traction force characteristics of migratory adenocarcinoma in heterogeneous type-1 collagen through bead-free confocal microscopy Public Deposited

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https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/cj82k8960

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  • Current methods in traction force analysis rely on a homogeneous extra-cellular matrix and embedded microbeads to track deformations. The efficacy of these methods decreases when the matrix is not homogeneous. In heterogeneous matrices, beads often aggregate in regions of higher matrix density and float in regions where matrix substrate is not present, making image analysis difficult. This study proposes a unique method of image analysis that does not require the presence of beads, and can be used to analyze the difference in deformation angles vs. distance from the cell membrane. This method established evidence for discontinuity in the mechanics of the matrix. In a heterogeneous matrix with large pore sizes, bending is heavily modulated by the fiber network, and does not follow a continuous 1/r law, where r is the distance from the cell.
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