The effects of follicle stimulating hormone dose administered to cattle embryo donors on embryo quality and in vitro development and plasminogen activator production Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/f7623f47x

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  • The relationship between dose of follicle stimulating hormone (FSH) used to superovulate cattle embryo donors and embryo viability as measured by in vitro development and plasminogen activator (PA) production was determined. Late morulae to blastocysts were collected non-surgically from 12 superovulated crossbred beef cows. Embryos were cultured for 8 d and at 24-h intervals embryos were evaluated for viability, stage of development and transferred to fresh culture medium. Conditioned medium was recovered and used for PA analysis. PA production was determined using a caseinolytic agar gel assay with human urokinase as the standard. Mean number of total ova and embryos recovered, transferrable embryos, degenerate embryos and unfertilized ova (UFOs) did not differ (P>0.10) between cows treated with 200 vs. 400 mg FSH. Likewise, mean percentage of embryos recovered, transferrable embryos, degenerate embryos and UFOs did not differ (P>0.10) between cows treated with 200 vs. 400 mg FSH. More embryos hatched (P<0.05) and developed sooner (P<0.05) to the expanded blastocyst stage recovered from cows superovulated with 200 mg compared to 400 mg FSH. Although not significantly different, PA production was twofold greater by embryos recovered from cows superovulated with 200 vs. 400 mg FSH. The poorer in vitro development observed by embryos recovered from cows treated with 400 mg FSH may be an indicator of in utero survival and the lower likelihood of pregnancy establishment.
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