Undergraduate Thesis Or Project
 

Developing a hemp dihaploid protocol

Öffentlich Deposited

Herunterladbarer Inhalt

PDF Herunterladen
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/f7623m67f

Descriptions

Attribute NameValues
Creator
Abstract
  • Cannabis production has gained popularity over the last 10 years. There is a growing demand for true-to-type grain, fiber, and resin crops. Breeding for homozygous trait development with traditional methods can take as long as 8 years. With dihaploid breeding, homozygosity can be achieved in 2 generations saving breeders time and effort. It may be possible to use androgenesis to culture microspores or immature pollen into sterile haploid plantlets. Then by using oryzalin or colchicine that inhibits the segregation of chromosomes during meiosis to create a 100% true-to-type fertile dihaploid that could be used to develop a homozygous cultivar. Currently there are few protocols to achieve doubled haploids for Cannabis sativa. The main research objective of this study was to identify key features in Cannabis sativa that correlate to successful production of doubled haploid plants. Some of these objectives included: confirming a way to quickly test pollen viability, identifying viable microspore stages from developing pollen grain, and determining whether silver thiosulfate (STS) induced males could produce viable tissue culture material. In studies on media that can induce pollen germination, it was determined that pollen viability through germination can be tested with Cannabis 1 media (Gaudet, 2020) with at least 10 varieties of hemp. Using the autoflower breeding line DNCBD-F3, it was found that anthers between 2.5-3.5mm appear to hold the highest density of early uninucleate microspores. Females of DNCBD-F3 feminized with 3mM STS (silver thiosulfate) produced viable microspores suitable for haploid plantlet production. In addition, the protocol for germination of Cannabis pollen demonstrated in this work is useful to test stored pollen viability for spatially and temporally separated breeding programs with Cannabis sativa.
Resource Type
Date Issued
Degree Level
Degree Name
Degree Field
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Urheberrechts-Erklärung
Publisher
Peer Reviewed
Language
File Format
File Extent
  • 18 pages

Beziehungen

Parents:

This work has no parents.

In Collection:

Artikel

Miniaturansicht Titel Hochladedatum Sichtbarkeit Aktionen
file details SeanKalaniMurphy2023.pdf 2023-06-06 Öffentlich Herunterladen