Honors College Thesis
 

Characterizing Rhythms in Circadian Gene Expression Following Chronic Binge-Like Alcohol Drinking in HDID Mice

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https://ir.library.oregonstate.edu/concern/honors_college_theses/ft848s34f

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  • Mammals exhibit circadian rhythms in several physiological processes controlled by a core set of circadian genes that make up the so-called molecular clock. Disruptions in these rhythms, and variations in circadian genes, are associated with the development of psychiatric disorders, including addiction. However, the effect of chronic binge-like alcohol drinking on rhythmic expression of circadian genes (such as Per2) in different brain regions is unknown. We focus here on two brain regions, the nucleus accumbens (NAc) – an area important for alcohol drinking, and the suprachiasmatic nucleus (SCN) – an area important in initiating and maintaining circadian rhythms. We used mice selectively bred to achieve high blood alcohol levels after a short drinking session (High Drinking in the Dark, HDID-1) and subjected them to an 8 week drinking in the dark (DID) protocol with either water or alcohol. After 8 weeks, the mice were euthanized, brain tissue was extracted at 8 time intervals and frozen to be processed for qPCR to quantify expression of Per2 in each region. The results show that alcohol disrupts rhythmic expression of circadian genes in these two regions, where the SCN is somewhat more resilient than the NAc to the effects of chronic binge-like drinking. Key Words: alcohol, circadian, gene, expression
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  • Portland VA, NIH
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