The diageotropica (dgt) mutation has been proposed to affect either auxin
perception or responsiveness in tomato plants. Auxins and cytokinins are plant
growth regulators known to interact with each other via a variety of mechanisms.
Here, we report the interactions of auxins and cytokinins in VFN8 wild-type and
dgt mutant seedling hypocotyl segments as relating to elongation, ethylene
production, and expression of four members of the auxin regulated Aux/IAA gene
family. The procedure differed from earlier studies in that the seedlings were
germinated and grown in the presence of cytokinin, rather than treated with
cytokinin just prior to auxin treatment. Conditional elongation and ethylene
production for both wild-type and dgt were similar to those previously reported.
With respect to auxin-induction, two of the genes tested for expression were
found at levels comparable to those previously described and two were found at
levels contrary to those previously described. To our knowledge, effects of
cytokinin on expression of these Aux/IAA gene family members has not been
previously examined. These results confirm that dgt plants have reduced
sensitivity to auxin, that cytokinin reduces this sensitivity further, and that wildtype
plants treated with cytokinin mimic only a subset of the effects of the dgt
mutation on known auxin-responses.
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