Undergraduate Thesis Or Project
 

Growth regulator interactions in the diageotropica : Mutant of Solanum lycopersicon

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https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/gh93h398f

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  • The diageotropica (dgt) mutation has been proposed to affect either auxin perception or responsiveness in tomato plants. Auxins and cytokinins are plant growth regulators known to interact with each other via a variety of mechanisms. Here, we report the interactions of auxins and cytokinins in VFN8 wild-type and dgt mutant seedling hypocotyl segments as relating to elongation, ethylene production, and expression of four members of the auxin regulated Aux/IAA gene family. The procedure differed from earlier studies in that the seedlings were germinated and grown in the presence of cytokinin, rather than treated with cytokinin just prior to auxin treatment. Conditional elongation and ethylene production for both wild-type and dgt were similar to those previously reported. With respect to auxin-induction, two of the genes tested for expression were found at levels comparable to those previously described and two were found at levels contrary to those previously described. To our knowledge, effects of cytokinin on expression of these Aux/IAA gene family members has not been previously examined. These results confirm that dgt plants have reduced sensitivity to auxin, that cytokinin reduces this sensitivity further, and that wildtype plants treated with cytokinin mimic only a subset of the effects of the dgt mutation on known auxin-responses.
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file details Canfield.pdf 2017-10-26 公开 下载