Many Gram-negative bacteria use a type III secretion system (TTSS) to establish a relationship with a host. Pathogens can use the secretion system as a way to deliver proteins known as type III effectors (TTEs) into a host cell and block host defense. The variation and redundancy of the type III effectors makes studying them a challenge. To address this challenge, we developed a stable delivery system for individual type III effectors into plant cells (EtHAn). The functionality of EtHAn was validated and later EtHAn was used to characterize individual type III effectors from the phytopathogen Pseudomonas syringae pv. tomato DC3000.