Undergraduate Thesis Or Project

 

The rate-based paper detection of vanillin and m-cresol using a colorimetric tyrosinase mediated chitosan-quinone reaction Public Deposited

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  • Monitoring the concentration of phenolic compounds is important for environmental regulation, health diagnostics, and bioenergy fermentation. Vanillin is a phenol compound used as a food flavoring that can cause allergies and inhibits biofuel production. The phenol m-cresol is a toxic compound that is regulated in the environment and in human health. A device can be created to semi-quantitatively determine the concentration of these compounds using a colored reaction that occurs when the phenol compounds are converted into quinones by the enzyme tyrosinase and then subsequently react with chitosan. As the concentration of phenol increases, the color intensity of the quinone-chitosan reaction product increases, allowing for a simple correlation between end point color intensity and phenol concentration. Since tyrosinase is an enzyme, its conversion rate increases with increased substrate. Therefor the rate of signal formation can also be used as a metric for determining the concentration of phenols present. The difference in rates at different concentrations can be used as another, more sensitive metric of concentration determination. It was found that vanillin could not be detected with the tyrosinase mediated quinone-chitosan reaction. m-Cresol was detected with the tyrosinase mediated quinone-chitosan reaction and a lower limit of detection was achieved by using the rate of signal generation as a metric over the end point concentration reading.
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  • This project is partially funded by AHB Education project supported by an Agriculture and Food Research Initiative Competitive Grant no. 2011-68005-30407 from the USDA National Institute of Food and Agriculture (NIFA).
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  • description.provenance : Approved for entry into archive by Steven Van Tuyl(steve.vantuyl@oregonstate.edu) on 2016-09-08T20:50:59Z (GMT) No. of bitstreams: 2license_rdf: 1223 bytes, checksum: d127a3413712d6c6e962d5d436c463fc (MD5)HinsonDylan2016.pdf: 459116 bytes, checksum: 23b9ccaa389da7f6dc0ea3c457aefb0f (MD5)
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