Antimicrobial Production in Burkholderia thailandensis Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/j098zc362

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  • Burkholderia thailandensis is a soil-dwelling bacterium that has been proven to produce antimicrobial compounds. We are interested in understanding how B. thailandensis regulates production of these compounds and also hope to identify and characterize novel bacterial factors that possess antimicrobial activity. In our research, we use a variety of methods including disc diffusion assays, competition experiments, and mutational analysis to investigate the genes responsible for the antimicrobial activity of B. thailandensis. The current research reports the presence of a previously undescribed diffusible antimicrobial factor produced by B. thailandensis. We have yet to identify the chemical nature of this factor or the genes responsible for its production. However, this factor is removed or inactivated by filter sterilization. We tested if cell-to-cell communication and a selection of candidate secondary metabolites are associated with this antimicrobial factor. The BtaR1 regulator for bacterial communication may control antimicrobial activity, but we do not believe it to be a different factor. Additionally, mutations in known BtaR1-controlled secondary metabolites do not influence antimicrobial activity against B. subtilis in our assays. Moving forward with this project we will continue to investigate the genes responsible for producing antimicrobial compounds by testing mutant strains of B. thailandensis. We hope to isolate and further characterize the antimicrobial factor(s).
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