Refinement of Absolute Quantification Mass Spectrometry Method to Detect and Monitor FMO levels in a Mouse Model of Tuberculosis

Undergraduate Thesis Or Project

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Citeable URL: https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/j38608266

Descriptions

Attribute Name	Values
Creator	Azevedo, Rachel
Abstract	The detection of mammalian FMO’s is important in the understanding of active FMO 2.1 and its role in metabolizing drugs to treat tuberculosis. Refinement of an absolute quantification (AQUA) mass spectrometry method is needed to be able to quantify the different FMO isoforms present in mammalian tissue. We are doing studies with wildtype and Fmo 1/2/4 null mice as a model for human FMO2.1 and FMO2.2, respectively and used model mouse Fmo peptides for our studies. Following denaturing polyacrylamide gel electrophoresis, an in-gel trypsin digestion was performed with labeled and unlabeled FMO proteins present. The samples were run on LC/MS/MS. Both the labeled and unlabeled FMO proteins from in-gel digestion were detected by LC/MS/MS along with a serial dilution of, directly injected, labeled FMO peptides.
Resource Type	Presentation
Date Available	2013-05-31T00:04:45+00:00
Date Issued	2012-09-16
Degree Level	Bachelor's
Degree Name	Bachelor of Science (B.S.)
Degree Field	Biology
Degree Grantor	Oregon State University
Commencement Year	2013
Advisor	Williams, David
Non-Academic Affiliation	Oregon State University. Undergraduate Research, Scholarship, and the Arts (URSA)
Declaração de direitos	Copyright Not Evaluated
Funding Statement (additional comments about funding)	Howard Hughes Medical Institute (HHMI); FMO grant: NIH/NHLBI PHS-HL038650
Publisher	Oregon State University
Language	English [eng]
Replaces	http://hdl.handle.net/1957/38798

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Miniatura	Título	Data de carga	Acesso	Ações
	Revised AQUA Presentation2A.pdf	07/21/2017	Público
	Revised AQUA Presentation2A.pptx	07/21/2017	Público