Cytokine expression in Danio rerio (zebrafish) in response to TLR-7 agonist - Imiquimod Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/k0698950w

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  • Utilizing Danio rerio (zebrafish) as a model organism has gained popularity recently, however a lack of research into their immune response pathways had led to the discovery of subclinical infections, and has potentially confounded results. We conducted a study to determine the cytokine expression from TLR activation in order to map these pathways and prevent further research complications. Zebrafish tissues were analyzed by flow cytometry for lymphocyte localization. Intestine, spleen and gill were exposed to an ssRNA analog imiquimod, bacterial flagellin, and an dsRNA analog poly(I:C). Fold-change of IFNγ1, IFNγ2, and TNFα were analyzed using real-time qPCR techniques. In response to imiquimod, intestine had the highest fold-change increases of IFNγ1 and IFNγ2 with 14.56 and 33.24 respectively, compared to gill (0.98, 0.58) and spleen (2.51, 7.50). Fold-change of IFNγ1 in response to flagellin was lower in spleen (3.83) and intestine (0.66) than to imiquimod (5.82, 9.35 respectively). Expression of IFNγ2 from flagellin-exposure was higher than imiquimod-exposure in spleen (7.50, 0.63), but lower in intestine (1.31, 8.23). Imiquimod incited the largest fold-change of IFNγ1 and IFNγ2 in the intestine. Since imiquimod is a TLR 7 agonist, the activity of TLR 7 in zebrafish may be related to proinflammatory antiviral immune response. Key Words: Cytokine, Immunobiology, Zebrafish, Toll-Like Receptor, TLR agonist
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2016-06-02T21:26:08Z (GMT) No. of bitstreams: 2license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5)PaternostroKevinA2016.pdf: 1092121 bytes, checksum: 8cf5dcf78aa8947db3a8529f0ecd8c43 (MD5)
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