Establishment of an in vitro model to study persistence in Mycobacterium avium subspecies hominissuis Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/k643b3269

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  • Mycobacterium avium subspecies hominissuis (MAH) is the most common pathogen among non-tuberculous mycobacteria, causing disease in immunocompromised individuals. An intracellular bacterium, MAH resides within the phagosome, a vesicle formed by macrophages as they engulf invading pathogens. Here, a subpopulation of MAH regresses into a nonreplicative state called persistence, allowing them to tolerate antimicrobial treatment. Unlike resistant bacteria, they are genetically identical to wild-type bacteria that cannot tolerate antibiotics. Persistent bacteria contribute to the development of chronic infection. Little is known about the mechanisms that allow MAH to lapse into this metabolic state. We investigated whether a previously established model with trace elemental mixtures representing the phagosome environment could be used to study persistence in MAH. We determined which mixture would yield the most persistent colony-forming units, then removed individual elements from full-spectrum mixture to determine which metals might be involved in inducing persistence. We established an in vitro model to study persistence in MAH based on media mimicking the phagosome environment after 24 hours of infection. Using this model, we found that iron might play a role in inducing persistence. Our model will enable researchers to study the mechanisms of persistence, contributing to current understanding of pathogenesis in MAH.
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  • description.provenance : Approved for entry into archive by Steven Van Tuyl(steve.vantuyl@oregonstate.edu) on 2017-01-23T17:33:51Z (GMT) No. of bitstreams: 1 ArmstrongElyssaL2017.pdf: 262181 bytes, checksum: ad36a12e4b6d14e1ff48800d5831c5bd (MD5)
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  • description.provenance : Submitted by Elyssa Armstrong (armstrel@oregonstate.edu) on 2017-01-10T22:15:47Z No. of bitstreams: 1 ArmstrongElyssaL2017.pdf: 262181 bytes, checksum: ad36a12e4b6d14e1ff48800d5831c5bd (MD5)

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