Next-generation sequencing of Coleomyia (Diptera: Asilidae) Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/m900nw503

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  • Coleomyia (Diptera: Asilidae) is a small genus of robber flies found in western North America, totaling eight described species. The goal of this project was to sequence the transcriptome and genome of Coleomyia. In the most recent phylogenetic analysis, it could not be placed in a recognized subfamily. Furthermore, there are only a few sequenced transcriptomes and no sequenced genomes for the family. Lastly, adult asilids are known to possess venom, a unique condition among Diptera. However, this venom has not been characterized, and the venom genes are unknown. Fresh specimens of Coleomyia setigera were collected and prepared for transcriptomic and genomic extraction. Libraries were subsequently prepared for Illumina sequencing. Raw sequences (reads) were assembled into contigs, then annotated using various programs. The assembled transcriptome was used, in conjunction with other sequence data, to infer the phylogeny of Asilidae. Assembly revealed that the genome was severely incomplete, while the transcriptome was mostly complete. The annotation identified 14 putative venom genes, although their role as venom in Asilidae is doubtful. The robber-fly phylogeny was unable to resolve the position of Coleomyia with strong support. The sequences produced in this study will aid future work in robber-fly systematics and venom characterization.
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