Photoluminescence Detection of 2,4,6-Trinitrotoulene (TNT) Binding on Single Cell Diatom Biosilica Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/np193c06t

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  • T. pseudonana biosilica and live cells was explored as a biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells of microscopic algae called diatoms. This material emits strong visible blue photoluminescence (PL) upon UV excitation. PL-active frustule biosilica was isolated from cultured cells of the marine diatom T. pseudonana, exposed to varying concentrations of TNT, and the PL response was observed using PL spectroscopy. This process was repeated for live T. pseudonana cells. Both experiments showed distinct trends and an attempt at explanation was made.
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