Undergraduate Thesis Or Project
 

EBB1, an AP2/ERF Transcription Factor, Promotes Transgenic Shoot Development in Populus

Público Deposited

Conteúdo disponível para baixar

Baixar PDF
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/nv935490k

Descriptions

Attribute NameValues
Creator
Abstract
  • Recalcitrance to transformation makes genetic engineering of many valuable plants infeasible for practical use. Transitory host modification during transformation using transgenes offers a possible means of overcoming this obstacle. In prior work in our laboratory, the genes GA20ox7 and EBB1 were found to increase regeneration in vitro. In this project, I reanalyzed the regenerative properties of these two genes using a model poplar (hybrid Populus tremula x P. alba INRA 717-1B4) and a genotype that is recalcitrant to transform, P. trichocarpa (Nisqually-1):N-1. Three replicate transformation experiments were performed to compare callus, shoot, and transgenic shoot formation with the two genes and an Empty-Vector control. No shoots were produced by N-1 explants with or without the genes. 717 explants transformed with p409S:GA20ox7 regenerated poorly, producing a low proportion of transgenic shoots for stem (12.4%) and leaf (8.19%) explants compared to that for empty vector controls (24.4%, 29.5%). 717 explants transformed with p409S:EBB1, however, had a greater numbers of shoots per stem and leaf explant (37.7% and 54.2% increase, respectively) and a higher proportion (50.8%) of transgenic shoots from 717 leaf explants (44.5%) compared to the Empty-Vector controls (29.5% ). Additionally, callus growth on N-1 stem explants was increased by 116% by EBB1 and 76.8% by GA20ox7 compared to Empty-Vector controls. I conclude that the EBB1 transgene shows promise in improving transformation, but it must be removed or reactivated shortly after transformation due to apparent cytokinin toxicity and morphological defects.  
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Non-Academic Affiliation
Declaração de direitos
Publisher
Peer Reviewed
Language
Replaces

Relações

Parents:
Em Collection:

Itens

Miniatura Título Data de carga Acesso Ações
file details J Ree Final Thesis 3 22 12.pdf 07/26/2017 Público
file details J Ree slides 3 15 12.pptx 07/26/2017 Público