Bacterial endophytes have the potential to confer benefits to Douglas-fir ( Pseudotsuga menziesii ), for instance drought tolerance. Bacterial endophytes originating from seeds are especially important. This is because seed endophytes are more likely to be the first endophytes to get established within the young host plant. The goals of this study were to characterize the bacterial seed endophytes of Douglas-fir from the Pacific Northwest and to test whether some bacterial seed endophytes can help seedlings survive under drought conditions. Using culture-based methods, endophytes were first isolated from 8 different populations of Douglas-fir (50 seeds per population). A total of 61 bacteria were isolated from seeds. Isolates were identified by sequencing the 16S rRNA region. A total of 7 genera were identified. Overall, the composition of bacterial seed endophytes differed among Douglas-fir populations, with Rahnella isolated most frequently. To test the hypothesis that bacterial seed endophytes could confer drought tolerance to Douglas-fir, two populations of seedlings (WA1 and CA2) were inoculated with Paenibacillus sp., Bacillus sp., or sterile water for control, then grown under drought conditions. Survival analysis revealed no significant effect of bacterial seed endophyte inoculation on seedling survival under drought conditions.