The response of cells to periodic driving is important for many biological processes, particularly blood flow and heart functioning. Our research analyzes the collective shear stress response of fibroblast cells due to a periodic driving frequency. When cells experience shear stress, they release calcium into the cytosol. To quantify the shear stress response of the cells, we use calcium imaging and single-cell level analysis. We had previously observed that the average collective calcium response of a monolayer of fibroblast cells had a different frequency than the applied periodic shear stress. Upon further testing, we ran into difficulties with air bubbles that caused the previous results to not be repeatable. We developed a new, more stable procedure including the use of a flow controller and a bubble trap. This work analyses the old procedure, the previous data, and the new procedure. With the changes made, we were able to successfully developed a new procedure to run the periodic shear stress tests.