Small secreted proteins of pyrenophora tritici-repentis and their role in wheat Infection Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/q237ht362

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  • Host-selective toxins (HSTs) secreted by the pathogenic isolate of Pyrenophora tritici-repentis, BFP, are major pathogenicity factors in tan spot disease of wheat. Along with characterized HSTs such as Ptr ToxA (ToxA), BFP secretes at least two more uncharacterized toxic components. In an attempt to identify these additional components, 12 candidate genes predicted by comparative genomics were further analyzed by comparing their gene expression with ToxA. Four candidate genes (PTRG-B, -C, -D, and -E) were identified and cloned. PTRG-C was successfully expressed using Pichia pastoris expression system and its protein product shown to be a secreted protein.
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