Kinetic analysis of the aerobic degradation of chlorinated ethenes by the mycobacterium ELW-1 and chlorinated ethanes and ethenes by rhodococcus rhodochrous ATCC® 21198™ Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/qf85nd02f

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  • Bioremediation of chlorinated ethenes by the augmentation of contaminated soils with microorganisms is a proposed method of reducing contaminant concentrations in groundwater. To determine the viability of using ELW-1 or R. rhodochrous for groundwater remediation, kinetic analyses of transformation rates must be performed. The focus of this research was to determine: 1) the specific rates of contaminant transformation when ELW-1 was exposed to chlorinated ethenes, 2) if R. rhodochrous has the ability to transform chlorinated ethenes and ethanes and 3) kinetic parameters of cometabolic processes using a modified Monod model to account for decrease in cell activity over time. Degradation rates for ELW-1 include 5.1 μmol/mg TSS-d for vinyl chloride (VC), 0.2 μmol/mg TSS-d for cis-Dichloroethene (c-DCE), and 4.1 μmol/mg TSS-d for trichloroethene (TCE). Chlorinated ethenes and ethanes were successfully degraded by R. rhodochrous with rates for VC, c-DCE, and TCE at 19.5 μmol/mg TSS-d 3.1 μmol/mg TSS-d and 0.1 μmol/mg TSS-d respectively. Transformation capacity analyses were performed with ELW-1 and TCE giving an estimated capacity of 0.04 mg TCE/mg TSS. A cometabolic transformation capacity model was also successfully fit to experimental data for the transformation of c-DCE by ELW-1.
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