Other Scholarly Content
 

Characterization of Gene Expression in Transgenic Plants with Modified Floral Gene Expression

Öffentlich Deposited

Herunterladbarer Inhalt

PDF Herunterladen
https://ir.library.oregonstate.edu/concern/defaults/qn59q531f

Descriptions

Attribute NameValues
Creator
Abstract
  • The release of transgenes into the environment by transgenic plant species is a significant ecological concern, and consequently a containment method for these genetically modified organisms is highly sought after. For poplar trees, an option for containment is to produce trees that do not flower and therefore cannot spread genetic material. The technique RNA interference (RNAi) is used to silence specific genes, and can be used in poplars to target floral developmental genes. Along with RNAi transgenes, the gene Heat Shock Promoter:FLOWERING LOCUS T (HSP:FT) is inserted into poplar trees to promote early flowering, allowing one to expedite the study of the effects of RNAi. Before one is able to study these effects, however, they must confirm that the poplars with HSP:FT insertion can successfully flower when the floral developmental genes are not targeted by RNAi, so that when RNAi transgenes are introduced, an absence of flowering can be attributed to RNAi rather than some other external factor. The goal of this study was to determine if there is a minimum level of FLOWERING LOCUS T gene (FT) expression required to induce flowering in poplar trees. Based on the results of this study, there appears to be a direct correlation between FT expression and flowering in poplar trees. The methods used in this study led to qualitative results that indicated only the presence or absence of FT expression, so the next step is to quantify the amount of FT present in the flowering poplar constructs using quantitative PCR (qPCR).
Contributor
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Advisor
Academic Affiliation
Non-Academic Affiliation
Urheberrechts-Erklärung
Funding Statement (additional comments about funding)
  • Oregon State University URISC Research Award; Oregon State University SURE Science Scholarship
Publisher
Peer Reviewed
Language
Replaces

Beziehungen

Parents:

This work has no parents.

In Collection:

Artikel

Miniaturansicht Titel Hochladedatum Sichtbarkeit Aktionen
file details SURE Project PowerPoint (Samantha Colby).pdf 07/21/2017 Öffentlich
file details SURE Project Description.pdf 07/21/2017 Öffentlich