Development and Characterization of Multiple Nanopore Systems for Molecular Sensing Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/qv33rz70f

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  • Application of Membrane Arrays to Detect DNA Through Simultaneous, Reversible Blocking Studies
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  • Artificial lipid bilayer technologies are effective models for studying cell membrane properties. Droplet bilayers rely on microfluidics and provide advantages over other bilayer methods due to their compatibility with automated development systems that increase yield while minimizing cost. This paper describes the implementation of bilayer technologies using membrane protein channels (α-hemolysin) combined with NeutrAvidin to detect DNA in multiple nanopore systems. The NeutrAvidin recognizes and binds a biotinylated DNA with high specificity, blocking the protein channel. This method has been used previously but here we substantially enhance reproducibility and detection capabilities by incorporating multiple nanopore systems with parallel arrays. Correlations between applied voltage, concentration, and number of ion channels were analyzed and predicted using a mathematical model in order to optimize the capabilities of these systems. The resulting relationship between the quantity of channels available and the time it takes to block has been shown to be critical when introducing an analyte into the system, as it provides a reference for analysis of various blocking events in multi-nanopore studies.
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