The broad spectrum antiviral compound ST-669 affects vesicular trafficking in Chlamydia-infected cells Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/rv042w054

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  • Our laboratory is exploring the mechanism of action of a novel broad spectrum antiviral, ST-669. This compound has activity against a variety of different viruses and the obligate intracellular bacterium, Chlamydia. In this study, we explored the effects of ST-669 when the cell cycle of the host cells was altered. Chlamydia spp. were grown in Vero cells in the presence of various compounds that inhibit the eukaryotic cell cycle, and examined for inclusion structure and production of bacteria. ST-669 was used to examine the inclusion structure of C. caviae: when treated with ST-669, C. caviae appears to fuse vacuoles to form an inclusion with a single lobe. Most of the cell cycle inhibitors did not alter the anti-chlamydial effects of ST-669. However, treatment of infected cells with vincristine led to an increase in bacteria production and change in inclusion morphology in the presence of ST-669. It is hypothesized that a protein that is a target of ST-669 is differently present or activated when Vero cells were treated with vincristine. These results open the door for future proteomic studies that might elucidate ST-669’s mechanism of action.
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