Honors College Thesis
 

Evaluation of Oxidation Reduction Potential in Metabolic Syndrome Subjects Compared with Matched Normal Subjects

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  • This study was conducted to assess whether healthy adults compared with those with Metabolic Syndrome (MetS) have different mean oxidative-reductive potentials. Static oxidation-reduction potentials and capacity oxidation reduction potentials (sORP and cORP, respectively) were measured in plasma samples. The RedoxSYS® measures sORP in millivolts (mV), which is proportional to the overall redox status of the sample, with higher sORP values relating to higher oxidation potential. The capacity of antioxidant reserves is measured by cORP in microcoulombs (μC), with lower values meaning the sample has below normal antioxidant reserve levels. Plasma from healthy men and women (n = 5 M, 5 F/group; age: 18-40 y; BMI: 22.6 ± 0.7 kg/m²) were compared with samples from age- and gender-matched individuals with MetS (n = 5 M, 5 F/group; age: 18-40 y BMI: 37.7 ± 3.0 kg/m²). Heparin-anticoagulated plasma samples, which were collected in the fasting state at 0, 24, 48, and 72 h from each of four separate trials, were used for study. The sORP (136.2±10.5 mV) measured in plasma from healthy subjects were significantly lower (P<0.0001) than in MetS subjects (154.5±19.2 mV, mean±SD). By contrast, the cORP measured in plasma from healthy subjects were approximately double those from MetS subjects (0.50±0.06 μC vs. 0.95±0.06 μC; respectively, P<0.0001). Spearman correlations were conducted to find if characteristics of MetS could be linked to sORP and cORP values. The combined values of BMI, HDL cholesterol, HOMA, and uric acid explained 93% of the variation in cORP. Seven variables (BMI, waist circumference, total and HDL cholesterol, CRP, TNFα, and γ-tocopherol) explained 91% of the variation in sORP. Assessment of ORP via this novel method appears to be a useful tool to rapidly assess metabolic status of a subject by using a fasting plasma sample. Higher sORP values appear to be a robust indicator of metabolic stress, while lower cORP values act as an indicator of lower metabolic resilience. Key Words: Metabolic Syndrome, RedoxSYS®, ORP, oxidative stress
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  • Luoxis’ RedoxSYS® Diagnostic System provided the 3-electrode strips and RedoxSYS as a gift to the lab.
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