Characterization of Fer1L6 in the mouse C2C12 cell line. Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/tb09j752q

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Fer1L6 is an uncharacterized member of the ferlin family of proteins, a group of large, calcium sensitive proteins with emerging roles in vesicle trafficking and various disease states. The presence of Fer1L6 has been identified within the C2C12 cell line of mouse skeletal muscle. To determine the protein’s subcellular location within the cell, we used immunofluorescence. Results indicated Fer1L6 localized to the plasma membrane and perinuclear region, specifically at lamellopodia and adhesion junctions. Co-immunofluoresence, proximity ligation, and co-immunoprecipitation experiments were then performed to identify any Fer1L6 binding partners so as to aid in elucidating the protein’s function. The proteins syntaxin-4, SNAP-23, vimentin, and actin were confirmed to bind Fer1L6. Additionally, the focal adhesion proteins vinculin and talin-1, were observed to be in close proximity to Fer1L6. This suggests that Fer1L6 may play a role in vesicle trafficking and membrane fusion at focal adhesions. Thus, Fer1L6 may function as a regulator in the recycling of the focal adhesion complex.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Advisor
Non-Academic Affiliation
Keyword
Rights Statement
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2015-05-15T16:47:18Z (GMT) No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) HuegelJacobT2015.pdf: 606687 bytes, checksum: b38066ef56329884c564078e5509ee1b (MD5)
  • description.provenance : Submitted by Jacob Huegel (huegelj@onid.orst.edu) on 2015-05-15T16:41:30Z No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) HuegelJacobT2015.pdf: 606687 bytes, checksum: b38066ef56329884c564078e5509ee1b (MD5)
  • description.provenance : Made available in DSpace on 2015-05-15T16:47:18Z (GMT). No. of bitstreams: 2 license_rdf: 1370 bytes, checksum: cd1af5ab51bcc7a5280cf305303530e9 (MD5) HuegelJacobT2015.pdf: 606687 bytes, checksum: b38066ef56329884c564078e5509ee1b (MD5)

Relationships

In Administrative Set:
Last modified: 07/28/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items