Undergraduate Thesis Or Project

Calibration of Laser Induced Fluorescence Thermometry using 2'7' Dichlorofluorescein and Sulforhodamine B

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  • An apparatus and method for calibrating planar laser induced fluorescence using a two-dye, two-color method was developed. A laser sheet was expanded from a 473 nm laser to excite dye solutions into higher energy states then fluoresced through spontaneous emission. The two fluorescent dyes selected for research and development were the primarily green emitting 2'7' dichlorofluorescein and the primarily red emitting sulforhodamine B. Planar laser induced fluorescence is used in thermal-fluids experiments as an accurate, non-invasive method for developing flow thermographs used to characterize the performance of experimental devices and methods. Two-color, two-dye methods are used because of their high accuracy, and potentially higher sensitivity relative to other thermography techniques because the temperature can be expressed as a function of only the fluorescence ratio of the two dyes and is independent of local laser intensity, which may vary considerably with time and space during an experiment. Calibration curves and relationships were developed that accurately correlated the local temperature of the solution to the intensity ratio of the dyes. Using a concentration of 2'7' Dichlorofluorescein of 4.624±0.294 × 10⁻⁶M and a concentration of Sulforhodamine B of 8.105±0.294 × 10⁻⁶M predicted temperature measurements had an uncertainty of 2.09 °C.
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Table of Contents
  • Table of Figures -- 2; Index of Equations -- 3; Abstract -- 4; Introduction -- 4; Laser Induced Fluorescence -- 4; Two Dye Two Color Method -- 5; Experimental Dyes -- 5; 2'7' Dichlorofluorescein (C20H10Cl2O5) -- 5; Sulforhodamine B (C27H30N2O7S2) -- 6; Methods -- 8; Experimental Set Up -- 8; Determining Dye Concentrations -- 11; Results -- 14; Analysis -- 18; Dye Concentration Error -- 18; Determining Calibration Curve -- 18; Conclusions -- 19; Acknowledgements -- 21; Works Cited -- 22;
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