Transient Electron Donor Concentration Experiments for the Determination of Dehalogenation Rate and Kinetic Parameter Shifts in an Anaerobic Microbial Culture Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/tt44pp60g

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  • Trichloroethene (TCE) is a common groundwater contaminant. Bioremediation, or the enhancement of natural microbial processes for the transformation of toxic compounds in soil and groundwater, is an effective solution to this widespread problem. The organisms that transform TCE in the subsurface are sensitive to environmental conditions, especially to the presence of an electron donor, which is required in the form of hydrogen (H₂) for the microbial processes to occur. This study explored the effects of electron donor concentration on TCE transformation rates of the anaerobic bacteria, Dehalococcoides mccartyi. Degradation rates of TCE and its transformation products were monitored for nine months following an increase in electron donor concentration within reactors maintaining mixed cultures of D. mccartyi at Oregon State University. Kinetic parameters representative of microbial transformation abilities were also monitored to develop conclusions regarding the community dynamics within the reactors. Key Words: Trichloroethene, bioremediation, Dehalococcoides, electron donor
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