Generation of multiple geneauxotrophs for use as selectable markers in Fusarium graminearum

Honors College Thesis

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Citeable URL: https://ir.library.oregonstate.edu/concern/honors_college_theses/w3763866x

Descriptions

Attribute Name	Values
Creator	Fargo, Corinne Marie
Abstract	Auxotrophic strains of the fungus Fusarium graminearum were generated in order to increase the number of functional selectable markers and improve the utility of the model organism. Single metabolic genes, the sequences encoding for enzymes required for the production of specific essential metabolites, were deleted in F. graminearum, and then validated strains were crossed to generate single strains with multiple metabolic gene deletions. To demonstrate the function and efficacy of these auxotrophic strains for future genetic analysis, single gene auxotrophs were complemented with the respective deficient metabolic gene as genetic markers to restore wildtype characteristics. Three separate F. graminearum strains with two essential gene deletions were generated and validated.
License	All rights reserved
Resource Type	Honors College Thesis
Date Available	2015-08-28T14:43:51+00:00
Date Issued	2015-08-25
Degree Level	Bachelor's
Degree Name	Honors Bachelor of Science (H.B.S.)
Degree Field	Biochemistry and Biophysics
Degree Grantor	Oregon State University
Commencement Year	2016
Advisor	Lancelin, Rebekah
Non-Academic Affiliation	Oregon State University. Honors College
Dichiarazione dei diritti	In Copyright
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Replaces	http://hdl.handle.net/1957/56981

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