Exposure to the Mycotoxin Zearalenone Impairs Embryonic Development in Zebrafish Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/w6634552t

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  • A study was conducted to evaluate the developmental toxicity of three commonly detected mycotoxins in food (deoxynivalenol, patulin, and zearalenone) using the zebrafish (Danio rerio) model. Mycotoxin exposures were carried out at five concentrations (0.0064, 0.064, 0.64, 6.4, and 64 μM). Morphological and photomotor screenings were conducted at 24 hours post fertilization (hpf) and five days post fertilization (dpf) to assess the effects of mycotoxin exposure on developing zebrafish embryos. No significant developmental toxicity was associated with embryonic exposure to deoxynivalenol or patulin. Incubation with 64 μM zearalenone was associated with significant incidences of mortality and adverse body morphology endpoints. Significant hypoactivity was associated with the 6.4 and 64 μM zearalenone exposures. Subsequently, a shuttle box assay was performed to determine the effects of incubation with 0.64 μM zearalenone on learning and recall in adult zebrafish, which were evaluated using active shock avoidance conditioning. Zebrafish incubated with 0.64 μM zearalenone recollected shock avoidance training at a slower rate than control fish, suggesting that embryonic zearalenone exposure may lead to adverse developmental outcomes in adult zebrafish. These results indicate that zearalenone may represent a developmental hazard, and that additional tests are warranted to further characterize the impacts of zearalenone exposure.
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