Adding value to renewable biodiesel production from the oleaginous yeast Lipomyces starkeyi Public

http://ir.library.oregonstate.edu/concern/honors_college_theses/w9505232k

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  • Global warming and on-going depletion of fossil fuels have stimulated research to develop renewable sources of energy. Current renewable energy use comprises only 10% of the energy consumed in the US and is focused mostly on generating electricity. The majority of the energy consumed by the US transportation sector is in the form of petroleum-based fuel. One potential renewable source is biodiesel, produced mainly from plant oil and animal fat. Production of biodiesel from plant oil competes with land use for food and feed crops. One alternative source of biodiesel that does not compete for agricultural land directly is oleaginous yeast, defined as yeast capable of producing >20% lipid as dry weight. This study tested a simplified approach for culturing the oleaginous yeast Lipomyces starkeyi 78-23 for lipid production, and tested a byproduct of the lipid extraction process for value as a substitute for commercial yeast extract. The lipid yield and composition were found to be comparable between cells grown on glucose or xylose. The aqueous phase recovered from the lipid extraction was tested as an alternative to commercial yeast extract in media used to culture S. cerevisiae S288c and L. starkeyi 78-23. On a weight basis, the extract was found to have about 4 times more nutritional value than commercial yeast extract for L. starkeyi and 3 time more nutritional value for S. cerevisiae.
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