Undergraduate Thesis Or Project
 

HHMI Presentation Transcript Notation.pptx

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  • We proposed to test the hypothesis that ecologically relevant concentrations of pesticide mixtures will increase susceptibility of metamorphic amphibians to a fungal pathogen known as Batrachochytrium dendrobatidis, or Bd. Bd has received considerable attention as one of the causes of declining amphibian populations. We hypothesized that exposure to the contaminants in the larval stage would cause increased pathogen load, decreased growth, and increased mortality of amphibians. Our amphibians included 2 species of tree frogs (Spring Peepers and Pacific tree frogs), 1 toad species (Western Toads), and 2 true frog species (Leopard frogs and Cascades frogs). Each species of frog was split into five different pesticide treatments: high herbicide, low herbicide, high insecticide, low insecticide, and a control treatment. Half of the frogs in each pesticide treatment were exposed as tadpoles and half were exposed as metamorphs. In the end, three of the five species showed significant mortality rates. We saw similarities between the tree frogs. They both showed a Bd effect with a significant p-value. The toads showed an increased susceptibility compared to the other species. Our true frogs showed very little mortality and had no significant effects.
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