Graduate Thesis Or Dissertation
 

A technique for the measurement of gibberellins and its application to the xylem sap of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]

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  • An understanding of the gibberellins present in Douglas-fir is essential to understanding and controlling the growth and development of this species. A technique was developed which permits the separation of certain gibberellins with high performance liquid chromatography and their identification with mass spectrometry. An extraction technique for the isolation of gibberellins from plant material was then developed. These techniques were demonstrated to be successful in identifying some of the gibberellins present in wild cucumber seeds, a plant system known to contain gibberellins, but no gibberellins were found in Douglas-fir xylem sap during the spring. However, an increase in growth inhibitor levels was observed which could have interfered with the detection of the gibberellins. The increase in the level of inhibitors during June and July coincided with the decrease in shoot growth, suggesting that the cessation of developmental processes is the result of an increased level of growth inhibitors rather than a change in concentration of free gibberellins. Another explanation for the lack of evidence for gibberellins in Douglas-fir xylem sap is that the native gibberellins may occur in a form other than the free acid and therefore could not be detected with procedures used in this investigation.
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