Graduate Thesis Or Dissertation

 

Basidiospores of Rhizopogon vinicolor and R. colossus as ectomycorrhizal inoculum Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/d791sj27n

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  • Basidiospores of Rhizopogon viriicolor Smith and R. colossus Smith were inoculated onto container-grown Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings and grown under two levels of soluble fertilizer and one level of slow-release fertilizer. Both fungi formed abundant (54%) ectomycorrhizae under the soluble fertilizer regimes. Slow-release fertilizer greatly reduced percent ectomycorrhizae for both fungi. Stern height was significantly increased under low fertility with all basidiospore application rates of R. colossus and the three lowest application rates of H. vinicolor. High fertility significantly increased ectomycorrhizae at all application rates of R. colossus. The HIGH fertility regime produced plantable Douglas-fir seedlings with abundant ectomycorrhizae of R. colossus and H. vinicolor. Five different conifers grown in a bareroot nursery were inoculated with three basidiospore rates of seven hypogeous ectomycorrhizal fungi. Two Douglas-fir seed sources were successfully inoculated with both R. vinicolor and R. colossus. For seedlings inoculated with B. vinicolor the HIGH basidiospore rate produced the most ectomycorrhizae on the greatest number of seedlings. For seedlings inoculated with R. colossus the HIGH basidiospore rate (seed source 062) or the MEDIUM rate (seed source 252) produced the most ectomycorrhizae on the greatest number of seedlings. No significant differences in stem heights or diameters could be detected between treatments. Bareroot nursery grown Douglas-fir noninoculated or inoculated with basidiospores of B. vinicolor were outplanted on a routine reforestation site formerly occupied by alder in southwestern Oregon. After two years, inoculated seedlings had significantly increased survival, stem height, stem diameter, and seedling biomass (PVI). Although new feeder roots of noninoculated and inoculated seedlings were colonized by indigenous fungi, B. vinicolor persisted on the old root system and colonized new feeder roots on inoculated seedlings.
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