Honors College Thesis
 

Genomic identification of candidate inclusion membrane proteins in Chlamydial species and microscopic analysis

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  • The Chlamydiaceae are a family of obligate intracellular bacteria with a unique biphasic developmental cycle. The replicating, metabolically active, intracellular phase bacteria (RBs) grow within a cellular vacuole know as the inclusion. Since the proteins the Chlamydiaceae secrete into this membrane are the means of host/parasite interaction, they have long been of interest. In 1995 IncA was the first to be identified as localized to the inclusion membrane, and many other Inc proteins have subsequently been discovered. It was shown that most Inc proteins have a unique bi-lobed hydrophobic domain approximately 40-80 amino acids long. This thesis used the recently published sequences of several Chlamydial species to identify potential Inc proteins and determine their relationship with established families of Incs in other species. Antibodies to one potential Inc were prepared, and florescent microscopy was used to confirm its localization to the inclusion membrane. Finally, possible future research is discussed.
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