Graduate Thesis Or Dissertation
 

Development of mucosal vaccines for avian influenza A virus composed of recombinant Lactococcus lactis and Streptococcus gordonii

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2514np76t

Descriptions

Attribute NameValues
Creator
Abstract
  • Novel mucosal vaccines (LL-M2e, LL-HAe, SG-HAe) were constructed from live, non-pathogenic Lactococcus lactis or Streptococcus gordonii that express conserved regions of HA or M2 antigens from avian influenza virus (AIV) A. All three vaccines evoked antigen-specific serum immunoglobulin G (IgG) responses in vaccinated chickens. The addition of the adjuvant cholera toxin B (CTB) increased the antigen specific IgG response. Fecal IgA and cell mediated immune responses were not detectable in the chickens. LL-M2e provided significant protection against lethal challenge compared to mock-vaccinated chickens. None of the chickens vaccinated with the control strain (LL-PIP) or mock-vaccine survived lethal challenge past 5 days, whereas 2 of the 12 chickens vaccinated with LL-M2e survived at least 7 days post-infection. An M2e-specific serum IgG concentration of 1100 ng/ml was found to be the minimum required for increased protection against AIV infection. SG-HAe, but not LL-HAe, provided a significant increase in the number of chickens that survived lethal challenge compared to control-vaccinated birds. However, no correlation between HAe-specific serum IgG and survival was found.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details ReeseKaleb2011thesis.pdf 2017-08-15 Public Download