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Curcumin induces human cathelicidin antimicrobial peptide gene expression through a vitamin D receptor-independent pathway

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dc.creator Guo, Chunxiao
dc.creator Rosoha, Elena
dc.creator Lowry, Malcolm B.
dc.creator Borregaard, Niels
dc.creator Gombart, Adrian F.
dc.date.accessioned 2012-04-10T20:45:47Z
dc.date.available 2012-04-10T20:45:47Z
dc.date.issued 2013-05
dc.identifier.citation Guo, C., Rosoha, E., Lowry, M. B., Borregaard, N., & Gombart, A. F. (2013). Curcumin induces human cathelicidin antimicrobial peptide gene expression through a vitamin D receptor-independent pathway. The Journal of Nutritional Biochemistry, 24(5), 754-759. doi:10.1016/j.jnutbio.2012.04.002 en_US
dc.identifier.uri http://hdl.handle.net/1957/28669
dc.description This is the authors' peer-reviewed accepted manuscript. The version of record is published and copyrighted by Elsevier and can be found at: http://www.elsevier.com/wps/find/journaldescription.cws_home/525013/description#description en_US
dc.description.abstract The vitamin D receptor (VDR) mediates the pleiotropic biologic effects of 1alpha,25 dihydroxy-vitamin D3. Recent in vitro studies suggested that curcumin and poly-unsaturated fatty acids (PUFAs) also bind to VDR with low affinity. As potential ligands for the VDR, we hypothesized that curcumin and PUFAs would induce expression of known VDR target genes in cells. In this study, we tested whether these compounds regulated two important VDR target genes - human cathelicidin antimicrobial peptide (CAMP) and 1,25-dihydroxyvitamin D3 24-hydroxylase (CYP24A1)- in human monocytic cell line U937, colon cancer cell line HT-29 and keratinocyte cell line HaCaT. We demonstrated that PUFAs failed to induce CAMP or CYP24A1 mRNA expression in all three cell lines, but curcumin up-regulated CAMP mRNA and protein levels in U937 cells. Curcumin treatment induced CAMP promoter activity from a luciferase reporter construct lacking the VDR binding site and did not increase binding of the VDR to the CAMP promoter as determined by chromatin immunoprecipitation assays. These findings indicate that induction of CAMP by curcumin occurs through a vitamin D receptor-independent manner. We conclude that PUFAs and curcumin do not function as ligands for the VDR. en_US
dc.description.sponsorship This study was supported by NIH grant 5R01AI65604 (A.F.G.). en_US
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Journal of Nutritional Biochemistry en_US
dc.relation.ispartofseries Vol. 24 no. 5 en_US
dc.subject innate immunity en_US
dc.subject vitamin D receptor en_US
dc.subject curcumin en_US
dc.subject poly-unsaturated fatty acid en_US
dc.subject cathelicidin en_US
dc.title Curcumin induces human cathelicidin antimicrobial peptide gene expression through a vitamin D receptor-independent pathway en_US
dc.type Article en_US


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