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Embryogenesis is dependent upon 12-lipoxygenase, 5-lipoxygenase, and α-tocopherol to modulate polyunsaturated fatty acid status and the production of oxidized fatty acids in zebrafish

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dc.contributor.advisor Traber, Maret G.
dc.creator Lebold, Katherine M.
dc.date.accessioned 2012-06-21T16:47:05Z
dc.date.available 2012-06-21T16:47:05Z
dc.date.copyright 2012-05-25
dc.date.issued 2012-05-25
dc.identifier.uri http://hdl.handle.net/1957/30036
dc.description Graduation date: 2012 en_US
dc.description.abstract Arachidonic acid (ARA) and docosahexaenoic acid (DHA) are polyunsaturated fatty acids required for proper embryonic development, specifically neurodevelopment. However, little is known regarding their conversion to other metabolites during embryogenesis. The oxidation of ARA gives rise to the biologically active eicosanoids and the oxidation of DHA gives rise to the biologically active docosanoids. The oxidation of ARA and DHA occurs through enzymatic processes, via lipoxygenase (LOX), or non-enzymatic processes, via radical-mediated lipid peroxidation. We hypothesize that oxidation of ARA and DHA via LOX is required for proper embryonic development. Additionally, we hypothesize that α-tocopherol, a potent lipid soluble antioxidant, mediates the conversion of ARA and DHA to their respective oxidized metabolites. Using zebrafish as a model of vertebrate embryogenesis, we found that the selective knockdown of either 12-LOX or 5-LOX decreased the production of docosanoids, altered fatty acid homeostasis, and increased the incidence of malformations and mortality in embryos by 24 hours post fertilization. α-Tocopherol deficiency also increased the incidence of malformations and mortality during embryogenesis, and in its absence, increased oxidized metabolites of ARA and DHA and decreased fatty acids concentrations. Therefore, oxidized metabolites of ARA and DHA perform crucial functions during embryonic development, but the production of oxidized fatty acids must be balanced with antioxidant bioavailability for proper embryogenesis. en_US
dc.language.iso en_US en_US
dc.subject Lipoxygenase en_US
dc.subject α-tocopherol en_US
dc.subject polyunsaturated fatty acid en_US
dc.subject Zebrafish en_US
dc.subject.lcsh Lipoxygenases -- Physiological effect en_US
dc.subject.lcsh Vitamin E -- Physiological effect en_US
dc.subject.lcsh Zebra danio -- Embryology en_US
dc.subject.lcsh Unsaturated fatty acids -- Physiological effect en_US
dc.subject.lcsh Vitamin E deficiency en_US
dc.title Embryogenesis is dependent upon 12-lipoxygenase, 5-lipoxygenase, and α-tocopherol to modulate polyunsaturated fatty acid status and the production of oxidized fatty acids in zebrafish en_US
dc.title Embryogenesis is dependent upon 12-lipoxygenase, 5-lipoxygenase, and alpha-tocopherol to modulate polyunsaturated fatty acid status and the production of oxidized fatty acids in zebrafish en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Nutrition en_US
dc.degree.level Master's en_US
dc.degree.discipline Health and Human Sciences en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Jump, Donald B.
dc.contributor.committeemember Tanguay, Robert L.
dc.contributor.committeemember Khanna, Sunil K.
dc.description.peerreview no en_us


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