Graduate Thesis Or Dissertation
 

Biologically inspired biosensors from fish chromatophores

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/j098zd92m

Descriptions

Attribute NameValues
Creator
Abstract
  • This thesis explores the feasibility of using melanophore-based biosensors from Oreochromis niloticus. Melanophores are one type of pigmented cell of the scales and fins of fish that respond in a motile fashion to a diverse range of stimuli. Fish scales were employed as the first step in determining the utility of melanophores as biosensors. Responsiveness of melanophores in scales was quantitated with several bioactive agents. Experiments with depolarizing potassium ion, guanethidine, yohimbine (an adrenergic antagonist), and capsaicin (a sensory stimulant) provided evidence that melanophores are under nervous regulation. Conditions were developed to allow simplification of intact scale preparations, entailing epidermal removal and dennervation of scales. This simplification resulted in increased sensitivity and responsiveness to a larger array of bioactive agents, including a cyclic AMP analog. The simplified preparation was successfully tested for its ability to function as a biosensor using pharmaceutical eye drops; the response observed was determined to be due to naphazoline, an adrenergic agonist. Methods were developed that enabled culturing of chromatophores independent from scales. Cultured chromatophores were found to be responsive to bioactive agents with a comparable degree of sensitivity as simplified scale preparations. Attempts were undertaken to develop co-cultures of chromatophores with other cell types and with further development melanophore-based biosensors can be exploited.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details DanoskyTonyaR1997.pdf 2017-08-15 Public Download