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Dietary zinc depletion and repletion affects plasma proteins: an analysis of the plasma proteome

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https://ir.library.oregonstate.edu/concern/articles/2v23vv279

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  • Zinc (Zn) deficiency is a problem world-wide. Current methods for assessing Zn status are limited to measuring plasma or serum Zn within populations suspected of deficiency. Despite the high prevalence of Zn deficiency in the human population there are no methods currently available for sensitively assessing Zn status among individuals. The purpose of this research was to utilize a proteomic approach using two-dimensional gel electrophoresis (2DE) and mass spectrometry to identify protein biomarkers that were sensitive to changes in dietary Zn levels in humans. Proteomic analysis was performed in human plasma samples (n = 6) obtained from healthy adult male subjects that completed a dietary Zn depletion/repletion protocol, current dietary zinc intake has a greater effect on fractional zinc absorption than does longer term zinc consumption in healthy adult men. Chung et al. (Am J Clin Nutr 87 (5):1224-1229, 2008). After a 13 day Zn acclimatization period where subjects consumed a Zn-adequate diet, the male subjects consumed a marginal Zn-depleted diet for 42 days followed by consumption of a Zn-repleted diet for 28 days. The samples at baseline, end of depletion and end of repletion were pre-fractionated through immuno-affinity columns to remove 14 highly abundant proteins, and each fraction separated by 2DE. Following staining by colloidal Coomassie blue and densitometric analysis, three proteins were identified by mass spectrometry as affected by changes in dietary Zn. Fibrin β and chain E, fragment double D were observed in the plasma protein fraction that remained bound to the immunoaffinity column. An unnamed protein that was related to immunoglobulins was observed in the immunodepleted plasma fraction. Fibrin β increased two-fold following the Zn depletion period and decreased to baseline values following the Zn repletion period; this protein may serve as a viable biomarker for Zn status in the future.
  • Keyword: Fibrin β, Two-dimensional gel electrophoresis, Marginal Zn deficiency, Zn biomarker, Dietary Zn depletion and repletion, Hemostasis
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  • Grider, A., Wickwire, K., Ho, E., Chung, C. S., & King, J. (2013). Dietary zinc depletion and repletion affects plasma proteins: An analysis of the plasma proteome. Biometals : An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine, 26(1), 133. doi: 10.1007/s10534-012-9600-4
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  • 26
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  • 1
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  • Supported, in part, by beef and veal producers and importers through their $1-per-head checkoffand was produced for the Cattlemen’s Beef Board and State Beef Councils by the National Cattlemen’s Beef Association; by the General Clinical Research Center, University of California, San Francisco at the San Francisco General Hospital; the National Center for Research Resources, National Institutes of Health (Grant MO1-RR00083-43); Oregon Agriculture Experiment Station (OR00735); the Environmental Health Science Center at Oregon State University (NIEHS P30 ES00210); USANA Health Sciences, Inc., Salt Lake City, UT and Georgia Agricultural Experiment Station (GEO00513).
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