Honors College Thesis
 

Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae

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  • Vibrio cholerae is the causative agent of cholera, a serious diarrheal disease in developing countries. V. cholerae has a unique redox driven respiration-linked sodium pump, Na⁺ translocating NADH:quinone oxidoreductase (NQR). Several reports previously showed that NQR plays an important role in virulence, metabolism, and sodium homeostasis of V. cholerae. This study aims to better understand the mechanisms of the effect of NQR on V. cholerae virulence, metabolism, and physiology. Analysis of the V. cholerae Δnqr mutant revealed that virulence gene expression is highly growth-phase dependent and virulence gene transcription is altered only at the early-log growth phase compared to the wild type. The Δnqr mutant also showed a broad range of metabolic defects, as assessed by a Phenotype Microarray™ (Biolog), suggesting the importance of NQR in the energy metabolism of V. cholerae. Furthermore, we also concluded that the effects of NQR on V. cholerae virulence gene expression were linked to respiration rather than sodium motive force. Taken together, these results suggest that NQR has broad growth dependent effects on V. cholerae metabolism and the observed changes in metabolism are linked to the observed changes in virulence gene expression following the loss of NQR.
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  • This work was supported by the Undergraduate Research, Innovation, Scholarship &Creativity (URISC) fund, the OSU Howard Hughes Medical Institute SummerUndergraduate Research Program, and grants from the National Institutes of Health toClaudia Häse (AI-063121-02).
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