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Determination of the Ergot Alkaloid Ergovaline in Tall Fescue Seed and Straw Using a QuEChERS Extraction Method with High-Performance Liquid Chromatography–Fluorescence Detection

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https://ir.library.oregonstate.edu/concern/articles/c247dx79q

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  • Ergovaline is an ergot alkaloid produced by the symbiotic endophyte Epichloë coenophiala which can colonize varieties of the cool-season grass tall fescue (Festuca arundinacea). It is the principle toxicant responsible for the vasoconstrictive and reproductive sequelae seen in “fescue toxicosis” in livestock which consume forage exceeding the threshold of toxicity established for this compound. A new method for extraction of ergovaline from tall fescue seed and straw was optimized and validated, based upon the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, with high performance liquid chromatography-fluorescence detection. Fourteen extraction solvents were tested; 2.1 mM ammonium carbonate: acetonitrile (50:50, v/v) had the highest and most consistent recovery (91-101%). Linearity, limit of detection, limit of quantitation, accuracy, intra- and inter-day precisions for tall fescue seed and straw were 100-3500 µg/kg; 37 and 30 µg/kg; 100 µg/kg; 98%; 3.0 and 1.6%; and 3.8 and 1.0%, respectively. When applied to 17 tall fescue straw samples, there was good agreement between the currently used solid phase extraction (SPE) and QuEChERS methods (correlation coefficient = 0.9978). The QuEChERS method achieved the goals of eliminating chlorinated solvents, and developing a fast, efficient, reliable method for quantitating ergovaline in tall fescue forage that can be applied in a high-throughput food safety laboratory.
  • Keywords: ergovaline, ergot alkaloids, HPLC-fluorescence, tall fescue
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  • Walker, K., Duringer, J., & Craig, A. M. (2015). Determination of the Ergot Alkaloid Ergovaline in Tall Fescue Seed and Straw Using a QuEChERS Extraction Method with High-Performance Liquid Chromatography–Fluorescence Detection. Journal of Agricultural and Food Chemistry, 63(16), 4236-4242. doi:10.1021/acs.jafc.5b01149
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  • 63
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  • 16
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  • Funding for this project was provided by the Oregon Agricultural Experiment Station (ORE00871) and the Oregon Export Straw Association.
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