Graduate Thesis Or Dissertation
 

Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/0k225d73h

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  • Bovine viral diarrhea virus (BVDV) is an enveloped, single-stranded positive-sense RNA virus. The BVDV genome is about 12.5 kb long and consists of a single open reading frame (ORF) flanked by untranslated regions at the 5' and 3' ends (5'-UTR and 3'- UTR, respectively). To investigate the role of cis-sequences of UTRs during BVDV replication, we designed four peptide-conjugated phosphorodiamidate morpholino oligomers(PPMO) that are complementary to nucleotides (nt) 10-33 (BVDV-10), 258-281 (BVDV- 258), 375-398 (BVDV-375), and 12503-12526 (BVDV-12503) of the BVDV genome. PPMO with sequences targeting nt 258-281 (BVDV-258), 375-398 (BVDV-375), and 12503-12526 (BVDV-12503) of the BVDV genome completely blocked BVDV replication in vitro, while the PPMO with sequence complementary to nt 10-33 (BVDV-10) did not have observable inhibition against BVDV virus replication. In addition, BVDV-375 and BVDV-12503 were able to inhibit virus replication at the viral RNA synthesis level. Our study demonstrated that the cis-sequences nt 258-281 and 375-398 in the 5’-UTR and nt 12503-12526 in the 3’-UTR are important for BVDV replication, while the cis-sequence nt 10-33 may not play a significant role during virus replication.
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