Graduate Thesis Or Dissertation
 

A descriptive analysis of the waterways in Coos Bay Oregon on the basis of general, ruminant and human Bacteroides-Prevotella 16S rDNA markers

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5425kd71q

Descriptions

Attribute NameValues
Creator
Abstract
  • We explain a new method of detecting non point source fecal contamination using a PCR based method called Touchdown Polymerase Chain Reaction (TD-PCR). Using genetic markers particular to general, ruminant and human Bacteroides- Prevotella genes, we identified presence in both fresh and salt water environments. Water samples from four sites were collected at approximate 2-week intervals for a year. Samples were analyzed for total coliforms, fecal coliforms, E. coli, and the presence of general, ruminant and human Bacteroides-Prevotella markers. We compared the odds of recovering each PCR marker between sites. We investigated the relationship between rainfall and recovery of PCR markers. Finally, we compared the sensitivity of the PCR methods to standard public health methods.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details JonesThomasEJr2003.pdf 2017-08-15 Public Download