Graduate Thesis Or Dissertation
 

Germination potential in monogerm seed of Beta vulgaris L.

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/9593tz61n

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  • The objective of this study was to investigate the low seed germination of 12 varieties of Oregon grown monogerm sugar beet, Beta vulgaris L., using several methods to determine the germination potential. The methods were: (1) X-ray radiograph examination, (2) standard laboratory germination following official rules, (3) laboratory germination by hydrogen peroxide method, and (4) field emergence. The X-ray technique was effective and accurate for determining the number of undeveloped seeds in the varieties examined. The use of this technique for determining germination potential is questionable at this time, since the abnormalities present in the seedballs could not be detected from the radiograph. The speed of germination, as well as total germination, was higher for the hydrogen peroxide method than the standard method. There was less difference between the two methods for those varieties having decorticated seed. The results of the hydrogen peroxide method compared more favorably with the field emergence results than did the standard method. The primary factors contributing to low laboratory germination were: (1) undeveloped seeds, (2) abnormal seedlings, and (3) firm ungerminated seeds. The undeveloped seed class included the completely empty seedball cavities and those seedballs having shrunken seeds. The abnormal seedlings were caused primarily by seed-borne pathogens and were most frequent in those varieties having natural seedballs. Abnormalities were higher when using the standard method than with the hydrogen peroxide method. The role of inhibitors, as determined by the number of firm ungerminated seeds, was minor for all the varieties except one. It was determined that of the three laboratory methods investigated, no single method would give an accurate estimate of the total germination potential of a variety. Therefore, either the X-ray technique or cutting should supplement one of the regular laboratory germination methods to gain additional information on the seedlot's potential.
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