Graduate Thesis Or Dissertation
 

Demonstration of a link between seminal plasma proteins and male fertility in the domestic fowl (Gallus domesticus)

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  • The objective of this research was to clarify the basis of subfertility in Delaware roosters. It was anticipated that a sensitive method would be needed to compare seminal plasma protein composition between subfertile and fertile roosters. Consequently, the applicability of two-dimensional electrophoresis was tested as a tool for the analysis of chicken seminal plasma proteins. Two-dimensional electrophoresis resolved 95 ± 4.4 derivative polypeptides from seminal plasma proteins of fertile roosters, whereas one-dimensional electrophoresis resolved only 23 ± 0.4. Thus, two-dimensional electrophoresis was found to be a useful tool for seminal plasma protein analysis. Seminal plasma composition was compared between subfertile Delaware and fertile roosters. Seminal plasma from subfertile roosters was characterized by an imbalance of proteins, electrolytes, and amino acids (P<0.05). Neither type of seminal plasma contained proteolytic activity. No difference (P>0.05) was observed in seminal plasma osmolality. Differences in seminal plasma composition were attributed to a dysfunction of the excurrent ducts of the testis. This realization lead to experiments designed to modulate subfertility. Hemicastration exacerbated (P<0.001) subfertility, whereas supplementation of spermatozoa with seminal plasma proteins from fertile roosters ameliorated (P<0.001) subfertility. Addition of seminal plasma proteins from subfertile roosters to spermatozoa from fertile roosters had no effect (P>0.05) on fertility. Therefore, subfertility was attributed to protein deficiency in seminal plasma rather than the presence of some agent that induces subfertility. The study of subfertile Delaware roosters has helped establish a link between seminal plasma proteins and fertility in the domestic fowl.
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