Graduate Thesis Or Dissertation
 

Drug resistance indexing Enterobacter as a method of identifying food contamination of public health significance

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  • The Enterobacter are often found in the feces of man and other warm blooded animals but have limited use as indicators of fecal contamination of foods and water since they are also often found as natural flora of non-fecal environments such as water and plants. This study proposes a method that may be useful for differentiating between fecal and non-fecal Enterobacter. Coliform bacteria associated with the human fecal environment often have a high incidence of multiple resistance to antibiotics. By determining multiple drug resistance and indexing Enterobacter in reference to their environmental source, it was discovered that isolates from human feces, raw sewage and certain foods demonstrated a higher index than isolates from rural soils, wild animal feces, cereal grains and other non-human environments. An index value greater than 0.2 indicated isolates from environments contaminated with human feces representing a high health risk while those isolates with an index of less than 0.2 came from relatively safe environments. Traditionally, the assessment of food borne health hazards is determined by measuring the quantity and kind of bacteria present. Drug resistance indexing will compliment these tests and aid in identifying serious bacterial contamination of foods.
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