Honors College Thesis
 

A (3-Aminopropyl)triethoxysilane (APTES) and Glutaraldehyde Functionalized Cellulose Surface for Immunoassays

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https://ir.library.oregonstate.edu/concern/honors_college_theses/6h4412497

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  • The detection of biomolecules is critical for clinical diagnostics but requires specialty labor and high cost. The use of microfluidic paper-based analytical devices (µPADs) for bioassays is simpler and cheaper but has low sensitivity. Improved immobilization of antibodies onto cellulose is demonstrated through surface functionalization with APTES and glutaraldehyde. The nature of this immobilization is explored through Attenuated Total Reflection Fourier-transform infrared spectroscopy (ATR-FTIR spectroscopy) and various washing procedures. The application of this novel immobilization technique is explored through antibodies conjugated with fluorescein isothiocyanate (FITC) in an indirect immunoassay for Rabbit Immunoglobulin G (Rabbit IgG). Compared to the unfunctionalized surface, the functionalized surface improves immobilization of antibodies and has comparable sensitivity in a fluorescent immunoassay.
  • Keywords: Microfluidics, paper-based analytical devices, immunoassay, surface chemistry
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