Honors College Thesis
 

Antibiotic Discovery in Soil Bacteria Isolated from Indonesian Black Water Ecosystems

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https://ir.library.oregonstate.edu/concern/honors_college_theses/c247dx74b

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  • Soil bacteria are known to be a prolific source of bioactive compounds. They produce secondary metabolites that can be used as therapeutic agents. This investigation focused on the chemistry and biology of secondary metabolites from two soil bacteria, Streptomyces herbaricolor ICBB 9409 and Streptomyces albiflaviniger ICBB 9297, which were isolated from soil samples collected from black water ecosystems on Borneo Island, Indonesia. Both strains were cultured in Modified Bennett’s medium, and their secondary metabolites were extracted using liquid-liquid extraction protocols using ethyl acetate. The extracts were then tested against five different pathogenic bacteria and a fungus by agar disc diffusion assay. Active compounds were then purified by chemical and bioassay guided fractionation experiments. Mass spectrometry and nuclear magnetic resonance spectroscopy experiments were used to elucidate their chemical structures. A new analogue of actinomycin was isolated and purified from the culture broth of ICBB 9409 strain. This compound has potent antibacterial activity against Staphylococcus aureus and Mycobacterium smegmatis. Furthermore, two novel analogues of elaiophylin, 2´-methyl- 11,11´-O-dimethylelaiophylin and 2,2´-dimethyl-11,11´-O-dimethylelaiophylin, were isolated from the culture broths of ICBB 9297.
  • Natural product discovery of novel elaiophylin analogues and actinomycin analogues.
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