Honors College Thesis
 

Development of micro-wire solid-state sensors for the real-time detection of metabolites at the Streptococcus mutans biofilm-biomaterial interface

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https://ir.library.oregonstate.edu/concern/honors_college_theses/df65v974k

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  • We have developed a 100 m diameter solid-state potentiometric pH sensor to study the bacterial metabolism at the biofilm-biomaterial interface in real-time. The newly developed agar-coated pH sensor is both highly resistant to biofouling and stable, with a drift of 1 mV/h in physiological conditions. We used multiwall carbon nanotubes (MWCNTs) as a back contact in this solid-state sensor which significantly improved the sensor response time to 0.5-1 s as well as near-Nernstian response with slopes of 50 mV/pH ± 5 mV/pH. We further positioned these sensors at the hard-to-reach interface of biofilm-dental composites to quantify the change in a chemical environment mediated by Streptococcus mutans (Sm) biofilm, a pathogenic lactic acid-producing oral bacterial species. The Sm biofilm was re-created by immobilizing the bacteria in a biocompatible hydrogel encapsulated structure, which was again placed on dental composites. Our systematic study showed that the lactic acid produced by Sm biofilm when exposed to 30 mM sucrose, in artificial saliva at 37oC, causes a pH shift from 6.0 to 5.5 at the biofilm-biomaterial interface; which is significant as tooth demineralization sets in at pH <5.5. Key Words: Streptococcus mutans, sensor, pH, hydrogel, ion-selective electrode, dendron
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